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FRET Energy Transfer

Förster Resonance Energy Transfer: non-radiative dipole-dipole coupling between donor and acceptor. Efficiency E = 1/(1+(r/R₀)⁶). R₀ depends on spectral overlap, orientation (κ²), and quantum yield.

R₀ (Förster): 4.9 nm
FRET Efficiency: 50%
Donor/Acceptor Ratio: 1.0